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T. Kim Dao, Kailey Ferger, J. David Lambert, A chitin-binding domain-containing gene is essential for shell development in the mollusc Tritia, Developmental Biology, Volume 520, 2025, Pages 1-12, ISSN 0012-1606, https://doi.org/10.1016/j.ydbio.2024.12.016. (https://www.sciencedirect.com/science/article/pii/S0012160624002884) Abstract: Mollusc shells are diverse in shape and size. They are created by a shell epithelium which secretes a chitinous periostracum membrane at the growing edge of the shell, and then coordinates biomineral deposition on the underside of this membrane. Although mollusc shells are important for studying the evolution of morphology, the molecular basis of the shell development is poorly understood. In this paper, we investigate genes involved in the shell development of the gastropod mollusc Tritia (previously known as Ilyanassa). We characterize the contributions of the 2d micromere to the shell and other non-shell structures. We identify eight shell-specific genes and five non-shell specific genes by comparing the transcriptomes of wild-type and 2d ablated embryos. Morpholino knockdown of one of the shell-specific genes, ToChitin-binding domain-containing (ToChitin BD), results in shell defects. The chitinous periostracal membranes in ToChitin BD morpholino knockdown embryos lose their well-defined edge and peroxidase gradient. 

Abstract Carpenter ants in the genus Camponotus are large, conspicuous ants that are abundant and ecologically influential in many terrestrial ecosystems. The bicolored carpenter ant, Camponotus vicinus Mayr, is distributed across a wide range of elevations and latitudes in western North America, where it is a prominent scavenger and predator. Here, we present a high-quality genome assembly of C. vicinus from a sample collected in Sonoma County, California, near the type locality of the species. This genome assembly consists of 38 scaffolds spanning 302.74 Mb, with contig N50 of 15.9 Mb, scaffold N50 of 19.9 Mb, and BUSCO completeness of 99.2%. This genome sequence will be a valuable resource for exploring the evolutionary ecology of C. vicinus and carpenter ants generally. It also provides an important tool for clarifying cryptic diversity within the C. vicinus species complex, a genetically diverse set of populations, some of which are quite localized and of conservation interest.